Abstract
Rifampin-resistant mutants were selected from UV-light-treated Bacillus cereus (20 mutants) and attenuated B. anthracis (23 mutants). In addition, spontaneous rifampin-resistant mutants were also isolated in attenuated B. anthracis (22 mutants). The rifampin resistance clusters of the rpoB gene were sequenced for all 65 mutants. Mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. The spontaneous rate of resistance was estimated at 1.57 x 10(-9) mutations/generation by a Luria-Delbrück fluctuation test.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacillus anthracis / drug effects
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Bacillus anthracis / genetics*
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Bacillus cereus / drug effects
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Bacillus cereus / genetics*
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DNA-Directed RNA Polymerases
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Drug Resistance, Bacterial / genetics*
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Mutation
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Plant Proteins / genetics*
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Rifampin / pharmacology*
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Sequence Homology, Amino Acid
Substances
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Plant Proteins
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RpoB protein, Oryza sativa
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DNA-Directed RNA Polymerases
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Rifampin