Abstract
Singlet oxygen (1O2), generated by irradiating methylene blue, is toxic to melanoma cell cultures. Hypotaurine is known to scavenge efficiently singlet oxygen; the addition of hypotaurine (800 microM) to the medium during irradiation of the dye produces a greater protective effect on cells than taurine added at the same concentration. The assay of some detoxifying enzymatic activities indicate a different mechanism of protection of the two molecules: taurine induces an efficient detoxifying enzymatic action with respect to the control; hypotaurine exerts its effect greatly by specifically scavenging singlet oxygen.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / pharmacology*
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Catalase / metabolism
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Cell Death
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Free Radical Scavengers / pharmacology*
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Glutathione Peroxidase / metabolism
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Glutathione Reductase / metabolism
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Humans
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Methylene Blue / pharmacology
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Photosensitizing Agents / pharmacology
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Singlet Oxygen / metabolism*
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Superoxide Dismutase / metabolism
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Taurine / analogs & derivatives*
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Taurine / pharmacology*
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Tumor Cells, Cultured
Substances
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Antioxidants
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Free Radical Scavengers
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Photosensitizing Agents
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Singlet Oxygen
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Taurine
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hypotaurine
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Catalase
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Glutathione Peroxidase
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Superoxide Dismutase
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Glutathione Reductase
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Methylene Blue