Hypotaurine protection on cell damage by singlet oxygen

Adv Exp Med Biol. 2000:483:157-62. doi: 10.1007/0-306-46838-7_16.

Abstract

Singlet oxygen (1O2), generated by irradiating methylene blue, is toxic to melanoma cell cultures. Hypotaurine is known to scavenge efficiently singlet oxygen; the addition of hypotaurine (800 microM) to the medium during irradiation of the dye produces a greater protective effect on cells than taurine added at the same concentration. The assay of some detoxifying enzymatic activities indicate a different mechanism of protection of the two molecules: taurine induces an efficient detoxifying enzymatic action with respect to the control; hypotaurine exerts its effect greatly by specifically scavenging singlet oxygen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Catalase / metabolism
  • Cell Death
  • Free Radical Scavengers / pharmacology*
  • Glutathione Peroxidase / metabolism
  • Glutathione Reductase / metabolism
  • Humans
  • Methylene Blue / pharmacology
  • Photosensitizing Agents / pharmacology
  • Singlet Oxygen / metabolism*
  • Superoxide Dismutase / metabolism
  • Taurine / analogs & derivatives*
  • Taurine / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Antioxidants
  • Free Radical Scavengers
  • Photosensitizing Agents
  • Singlet Oxygen
  • Taurine
  • hypotaurine
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Reductase
  • Methylene Blue