[Collagen-binding proteins of human oral spirochetes]

Zhonghua Kou Qiang Yi Xue Za Zhi. 1999 May;34(3):165-7.
[Article in Chinese]

Abstract

Objective: To evaluate pathogenic effect of the collagen-binding proteins(CBP) of human oral spirochetes.

Methods: Immunoblot analysis, immunoelectron microscopy and other experiments had been used.

Results: It was showed that: type I, IV and V CBP were detected in heated and unheated preparations from Treponema denticola(Td) ATCC 33520 and Treponema socranskii (Ts) ATCC 35534. Few CBP, however, were detected in heated and unheated preparations from a recently characterized isloate, Treponema medium(Tm) strain G7201. 27,000 type V CBP of Td, 110,000 type I of Ts and 95,000 type IV of Tm were located on the envelopes of the individual cells. The adherence of Td to the collagen-coated surface was significantly greater than that of Tm.

Conclusion: The CBP on the oral spirochetal cells play an important role in their adherence to collagen-rich connective tissues of the host.

Publication types

  • English Abstract

MeSH terms

  • Bacterial Adhesion
  • Bacterial Proteins / metabolism
  • Collagen Type I / metabolism
  • Collagen Type IV / metabolism
  • Collagen Type V / metabolism
  • Humans
  • Integrins / metabolism*
  • Mouth / microbiology
  • Protein Binding
  • Receptors, Collagen
  • Species Specificity
  • Spirochaetales / chemistry*
  • Spirochaetales / physiology
  • Treponema / chemistry
  • Treponema / physiology

Substances

  • Bacterial Proteins
  • Collagen Type I
  • Collagen Type IV
  • Collagen Type V
  • Integrins
  • Receptors, Collagen