[Experimental study of Bcl-2 and Fas gene expression in fibroblast of scar]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2001 Nov;15(6):351-3.
[Article in Chinese]

Abstract

Objective: To explore the effect of Fas/Apo-1 and Bcl-2 gene expression on mechanism of scar formation.

Methods: Immunohistochemical method was applied to defect the expression of Fas and Bcl-2 protein in fibroblasts from 10 cases with normal skin, 10 cases with hypertrophic scar and 10 cases with keloid.

Results: The positive expression rate of Bcl-2 protein in keloid was 83.2%, significantly higher than that in hypertrophic scar (38.6%), (P < 0.01), and the positive expression rate in hypertrophic scar and keloid was higher than that in normal skin (6.78%), (P < 0.01). But the positive expression rate of Fas/Apo-1 protein was 78.4% in normal skin 80.4% in hypertrophic scar, 84.4% in keloid respectively, which showed no significant difference among them (P > 0.05).

Conclusion: Bcl-2 gene but Fas gene may take part in the formation of pathologic scar.

MeSH terms

  • Adolescent
  • Adult
  • Apoptosis / genetics*
  • Child
  • Child, Preschool
  • Cicatrix, Hypertrophic / genetics
  • Cicatrix, Hypertrophic / metabolism*
  • Cicatrix, Hypertrophic / pathology
  • Female
  • Fibroblasts / metabolism*
  • Fibroblasts / pathology
  • Gene Expression
  • Humans
  • Keloid / metabolism
  • Male
  • Middle Aged
  • Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Skin / metabolism
  • Skin / pathology
  • fas Receptor / biosynthesis*
  • fas Receptor / genetics

Substances

  • Proto-Oncogene Proteins c-bcl-2
  • fas Receptor