Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene

AIDS. 2002 Jan 4;16(1):123-5. doi: 10.1097/00002030-200201040-00016.

Abstract

HIV-1(HXB2) 5'LTR region, most of BIV(R29) gag-pol segment and HIV-1(HXB2) pol IN-3'LTR region were respectively amplified. A chimeric clone, designated as pHBIV(3753), was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines*
  • Animals
  • Cattle
  • Cell Line
  • Genes, gag / genetics*
  • Genes, pol / genetics*
  • HIV Long Terminal Repeat / genetics
  • HIV-1 / genetics*
  • HIV-1 / metabolism
  • HIV-1 / physiology
  • Humans
  • Immunodeficiency Virus, Bovine / genetics*
  • Immunodeficiency Virus, Bovine / metabolism
  • Immunodeficiency Virus, Bovine / physiology
  • Recombinant Fusion Proteins / genetics*
  • Recombinant Fusion Proteins / metabolism
  • Transfection

Substances

  • AIDS Vaccines
  • Recombinant Fusion Proteins