In vitro evaluation of a chitosan membrane cross-linked with genipin

J Biomater Sci Polym Ed. 2001;12(8):835-50. doi: 10.1163/156856201753113051.

Abstract

The study was to evaluate the characteristics of a chitosan membrane cross-linked with a naturally-occurring cross-linking reagent, genipin. This newly-developed genipin-cross-linked chitosan membrane may be used as an implantable drug-delivery system. The chitosan membrane without cross-linking (fresh) and the glutaraldehyde-cross-linked chitosan membrane were used as controls. The characteristics of test chitosan membranes evaluated were their cross-linking degree, swelling ratio, mechanical properties. antimicrobial activity, cytotoxicity, and degradability. It was found that cross-linking of chitosan membrane using genipin increased its ultimate tensile strength but significantly reduced its strain-at-fracture and swelling ratio. There was no significant difference in antimicrobial activity between the genipin-cross-linked chitosan membrane and its fresh counterpart. Additionally, the results showed that the genipin-cross-linked chitosan membrane had a significantly less cytotoxicity and a slower degradation rate compared to the glutaraldehyde-cross-linked membrane. These results suggested that the genipin-cross-linked chitosan membrane may be a promising carrier for fabricating an implantable drug-delivery system. The drug-release characteristics of the genipin-cross-linked chitosan membrane are currently under investigation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesives
  • Animals
  • Bacteria / drug effects
  • Bacteria / growth & development
  • Biocompatible Materials*
  • Cell Survival / drug effects
  • Chickens
  • Chitin / analogs & derivatives
  • Chitin / chemistry*
  • Chitin / toxicity
  • Chitosan
  • Colony-Forming Units Assay
  • Cross-Linking Reagents
  • Drug Carriers
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Glutaral / chemistry
  • Humans
  • Iridoid Glycosides
  • Iridoids
  • Membranes, Artificial*
  • Muramidase / metabolism
  • Pyrans / chemistry*
  • Pyrans / toxicity

Substances

  • Adhesives
  • Biocompatible Materials
  • Cross-Linking Reagents
  • Drug Carriers
  • Iridoid Glycosides
  • Iridoids
  • Membranes, Artificial
  • Pyrans
  • Chitin
  • Chitosan
  • genipin
  • Muramidase
  • Glutaral