Compaction, stainability and methylation of the late replicating X chromosome in mouse female fibroblasts

Cytogenet Cell Genet. 2001;94(1-2):79-81. doi: 10.1159/000048787.

Abstract

In metaphases from female mouse fibroblasts, successive stainings by Giemsa and DAPI and immunolabeling of 5-methylcytosine were performed with or without bromodeoxyuridine pretreatment. It was shown that, compared to all other chromosomes, the late replicating X is the least methylated, the most compacted, and the most intensely stained by DAPI and Giemsa.

MeSH terms

  • 5-Methylcytosine
  • Animals
  • Azure Stains / metabolism
  • Bromodeoxyuridine
  • Chromatin / chemistry
  • Chromatin / genetics
  • Chromatin / metabolism
  • Cytosine / analogs & derivatives*
  • Cytosine / metabolism
  • DNA / biosynthesis
  • DNA / chemistry
  • DNA / metabolism
  • DNA Methylation*
  • DNA Replication*
  • Female
  • Fibroblasts / metabolism*
  • Indoles / metabolism
  • Metaphase
  • Molecular Conformation
  • Staining and Labeling*
  • X Chromosome / chemistry*
  • X Chromosome / genetics
  • X Chromosome / metabolism*

Substances

  • Azure Stains
  • Chromatin
  • Indoles
  • DAPI
  • 5-Methylcytosine
  • Cytosine
  • DNA
  • Bromodeoxyuridine