Antisense properties of 2'-O-dimethylaminooxyethyl (2'-O-DMAOE) oligonucleotides

T P Prakash; A M Kawasaki; J F Johnston; M J Graham; T P Condon; M Manoharan

doi:10.1081/NCN-100002439

Antisense properties of 2'-O-dimethylaminooxyethyl (2'-O-DMAOE) oligonucleotides

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):829-32. doi: 10.1081/NCN-100002439.

Authors

T P Prakash¹, A M Kawasaki, J F Johnston, M J Graham, T P Condon, M Manoharan

Affiliation

¹ Isis Pharmaceuticals, 2292 Faraday Ave., Carlsbad, California 92008, USA.

PMID: 11563125
DOI: 10.1081/NCN-100002439

Abstract

Antisense oligonucleotides with 2'-O-(2-[N,N-dimethyl)aminooxy]ethyl) or (2'-O-DMAOE) modification were synthesized and evaluated for nuclease resistance and pharmacology both in vitro and in vivo. This modification exhibits very high nuclease resistance and efficacy in various biological (ICAM-1, C-raf and PKC-alpha) targets.

MeSH terms

Animals
Cells, Cultured
Endothelium, Vascular / drug effects
Endothelium, Vascular / metabolism
Female
Humans
Intercellular Adhesion Molecule-1 / biosynthesis
Intercellular Adhesion Molecule-1 / genetics
Mice
Mice, Inbred BALB C
Oligonucleotides, Antisense / chemical synthesis*
Oligonucleotides, Antisense / genetics
Oligonucleotides, Antisense / pharmacology*
Organophosphorus Compounds / chemical synthesis
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
Ribonuclease H / genetics

Substances

Oligonucleotides, Antisense
Organophosphorus Compounds
RNA, Messenger
phosphoramidite
Intercellular Adhesion Molecule-1
Ribonuclease H