Extra stable 2',5'-linked RNA loops

R N Hannoush; M J Damha

doi:10.1081/NCN-100002519

Extra stable 2',5'-linked RNA loops

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):1201-4. doi: 10.1081/NCN-100002519.

Authors

R N Hannoush¹, M J Damha

Affiliation

¹ Department of Chemistry, McGill University, Montreal, QC, Canada H3A2K6.

PMID: 11562986
DOI: 10.1081/NCN-100002519

Abstract

We prepared hairpins that differ in the connectivity of phosphodiester linkages in the loop (RNA vs 2', 5'-RNA). We find that the stability of the extra stable RNA hairpin 5'-rGGAC(UUCG)GUCC-3' is the same as that observed for the hairpin containing a 2',5'RNA loop, i.e. 5'-rGGAC(UUCG)GUCC-3' (where UUCG = U2'p5'U2'p5' C2'p5'G2'p5'). Also significant is the finding that when the stem is duplex DNA, duplex 2',5'-RNA, or DNA:2',5'-RNA, hairpins with the UUCG loop are more stable than those with UUCG loop.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Nucleic Acid Conformation
RNA / chemistry*
Thermodynamics

Substances

RNA