Kinetics study of the biotransformation of an oligonucleotide prodrug in cells extract by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):1159-63. doi: 10.1081/NCN-100002510.

Abstract

The fate of a dodecathymidine prodrug in cell extract was monitored by MALDI-TOF MS. This technique allows a facile identification and a relative quantification of metabolites produced. We showed that the relative peak intensities were similar to the relative metabolite proportions that permitted the determination of their half-lives. The oligonucleotide prodrug was fully metabolized to yield the T12 phosphorothioate likely through a carboxyesterase mediated mechanism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotransformation
  • Oligonucleotides / pharmacokinetics*
  • Prodrugs / pharmacokinetics*
  • Pyrimidine Nucleotides / pharmacokinetics*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Thymidine / analogs & derivatives*

Substances

  • Oligonucleotides
  • Prodrugs
  • Pyrimidine Nucleotides
  • Thymidine