Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A

Bioorg Med Chem Lett. 2001 Sep 17;11(18):2545-8. doi: 10.1016/s0960-894x(01)00494-2.

Abstract

Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coenzyme A / chemical synthesis*
  • Coenzyme A / chemistry
  • Coenzyme A / metabolism
  • Humans
  • Magnetic Resonance Spectroscopy
  • Mixed Function Oxygenases / chemistry
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism*
  • Phytanic Acid / analogs & derivatives*
  • Phytanic Acid / chemical synthesis*
  • Phytanic Acid / chemistry
  • Phytanic Acid / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Spectrometry, Mass, Electrospray Ionization
  • Stereoisomerism

Substances

  • Recombinant Proteins
  • hydroxyphytanoyl-coenzyme A
  • phytanoyl-coenzyme A
  • Phytanic Acid
  • Mixed Function Oxygenases
  • PHYH protein, human
  • Coenzyme A