Abstract
We quantitatively evaluate the changes of the proliferative cell populations in the adult tench retinas maintained at 6 degrees C and 20 degrees C by both PCNA antigen detection and flow cytometry-based DNA measurements. Both the overall percentage of S-phase cells in the whole retinas and the number of PCNA-positive cells in each of the retinal layers were significantly lower in the tench kept at 6 degrees C, indicating that temperature affects the retinal germinal cell proliferation.
MeSH terms
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Animals
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Body Temperature / physiology*
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Cell Differentiation / physiology
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Cell Division / physiology*
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Cell Lineage / physiology
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Cell Size / physiology
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Cyprinidae / anatomy & histology
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Cyprinidae / growth & development*
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Cyprinidae / metabolism
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DNA / metabolism
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Flow Cytometry
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Immunohistochemistry
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Models, Animal
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Nerve Regeneration / physiology
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Neuroglia / cytology
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Neuroglia / metabolism
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Neurons / cytology
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Neurons / metabolism*
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Photoreceptor Cells / cytology
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Photoreceptor Cells / metabolism
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Proliferating Cell Nuclear Antigen / metabolism
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Retina / cytology
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Retina / growth & development*
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Retina / metabolism
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Retinal Ganglion Cells / cytology
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Retinal Ganglion Cells / metabolism
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Stem Cells / metabolism*
Substances
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Proliferating Cell Nuclear Antigen
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DNA