Dehalogenation of chlorinated hydroxybiphenyls by fungal laccase

Appl Environ Microbiol. 2001 Sep;67(9):4377-81. doi: 10.1128/AEM.67.9.4377-4381.2001.

Abstract

We have investigated the transformation of chlorinated hydroxybiphenyls by laccase produced by Pycnoporus cinnabarinus. The compounds used were transformed to sparingly water-soluble colored precipitates which were identified by gas chromatography-mass spectrometry as oligomerization products of the chlorinated hydroxybiphenyls. During oligomerization of 2-hydroxy-5-chlorobiphenyl and 3-chloro-4-hydroxybiphenyl, dechlorinated C---C-linked dimers were formed, demonstrating the dehalogenation ability of laccase. In addition to these nonhalogenated dimers, both monohalogenated and dihalogenated dimers were identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basidiomycota / enzymology*
  • Basidiomycota / growth & development
  • Biodegradation, Environmental
  • Biphenyl Compounds / chemistry
  • Biphenyl Compounds / metabolism*
  • Gas Chromatography-Mass Spectrometry
  • Laccase
  • Magnetic Resonance Spectroscopy
  • Oxidoreductases / metabolism*
  • Polychlorinated Biphenyls / chemistry
  • Polychlorinated Biphenyls / metabolism*

Substances

  • Biphenyl Compounds
  • Polychlorinated Biphenyls
  • Oxidoreductases
  • Laccase