A study on the expression of interleukin (IL)-10 and IL-12 P35, P40 mRNA in the psoriatic lesions

J Tongji Med Univ. 2001;21(1):86-8. doi: 10.1007/BF02888047.

Abstract

To investigate the possible role of interleukin (IL)-10 and IL-12 in the pathogenesis of psoriatic lesions and to supply theoretical basis for the gene therapy for psoriasis, the expression of IL-10 and IL-12 P35, P40 mRNA in 12 cases of psoriatic lesions and 6 normal skin tissues was detected by using RT-PCR technique. The results showed that the expression of IL-10 mRNA in the psoriatic lesions was significantly lower than that in the normal skin tissues (P < 0.001). The expression of IL-12 P35 was positive both in the psoriatic lesions and in the normal skin tissues. IL-12 P40 mRNA was expressed positively only in the psoriatic lesions but negatively in the normal skin tissues. It was suggested that IL-12 might take an important role in the occurrence and progression of psoriasis, but IL-10 might have certain role in the regression of psoriasis.

MeSH terms

  • Adult
  • Female
  • Humans
  • Interleukin-10 / biosynthesis*
  • Interleukin-10 / genetics
  • Interleukin-12 / biosynthesis*
  • Interleukin-12 / genetics
  • Interleukin-12 Subunit p35
  • Interleukin-12 Subunit p40
  • Male
  • Middle Aged
  • Protein Subunits / biosynthesis*
  • Protein Subunits / genetics
  • Psoriasis / metabolism*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • IL12A protein, human
  • Interleukin-12 Subunit p35
  • Interleukin-12 Subunit p40
  • Protein Subunits
  • RNA, Messenger
  • Interleukin-10
  • Interleukin-12