On the basis of established nude mice transplanted tumor model of human tongue squamous cell carcinoma, peritumor injection was performed with 3H-TdR labeled TIL from human tongue cancer after 4 weeks culture. The dynamic distribution of 3H-TIL in vivo was learned through detecting the radioactivity of the target tissues. The results demonstrated that 3H-TIL was found in the whole body. There were about 2.1-30 times more in tumor tissues than in normal tissues and 3H-TILs even collected in the tumor tissues distal to the injective sites. The nearer to the injective sites the tumor tissues, the more 3H-TIL collected. It showed that local injection of TIL could be expected to exert a better antitumor effect.