Recombinant expression of proteoglycans in mammalian cells. Utility and advantages of the vaccinia virus/T7 bacteriophage hybrid expression system

Methods Mol Biol. 2001:171:201-19. doi: 10.1385/1-59259-209-0:201.
No abstract available

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacteriophage T7 / genetics*
  • Base Sequence
  • Cell Line
  • Chromatography, Affinity
  • DNA, Recombinant / genetics
  • DNA-Directed RNA Polymerases / genetics
  • Gene Expression
  • Genetic Vectors
  • Humans
  • Mammals
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Proteoglycans / biosynthesis*
  • Proteoglycans / genetics*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Recombination, Genetic
  • Transfection
  • Vaccinia virus / genetics*
  • Viral Plaque Assay
  • Viral Proteins
  • Virus Cultivation

Substances

  • DNA, Recombinant
  • Proteoglycans
  • Recombinant Proteins
  • Viral Proteins
  • bacteriophage T7 RNA polymerase
  • DNA-Directed RNA Polymerases