Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates

E Bart-Delabesse; J Sarfati; J P Debeaupuis; W van Leeuwen; A van Belkum; S Bretagne; J P Latge

doi:10.1128/JCM.39.7.2683-2686.2001

Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates

J Clin Microbiol. 2001 Jul;39(7):2683-6. doi: 10.1128/JCM.39.7.2683-2686.2001.

Authors

E Bart-Delabesse¹, J Sarfati, J P Debeaupuis, W van Leeuwen, A van Belkum, S Bretagne, J P Latge

Affiliation

¹ Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor, Créteil, France.

Abstract

Aspergillus fumigatus fingerprints generated by random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) upon hybridization with repeated DNA sequences, and PCR detection of microsatellite length polymorphism (MLP) were compared among 67 isolates. In contrast to RAPD, RFLP and MLP gave discriminating and significantly concordant genotyping results.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Aspergillosis / microbiology
Aspergillus fumigatus / classification*
Aspergillus fumigatus / genetics*
DNA Fingerprinting / methods
DNA, Fungal / analysis
DNA, Fungal / genetics
Environmental Microbiology
Humans
Microsatellite Repeats / genetics*
Mycological Typing Techniques
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length*
Random Amplified Polymorphic DNA Technique*

Substances

DNA, Fungal