Subtyping of MRSA isolates belonging to a widely disseminated clonal group by polymorphism of the dru sequences in mec-associated DNA

Int J Med Microbiol. 2001 Apr;291(1):57-62. doi: 10.1078/1438-4221-00116.

Abstract

During the past 7 years the "Berlin epidemic MRSA" has spread throughout whole Germany. SmaI macrorestriction patterns of this clonal group are rather stable as are the length polymorphisms at the 3' end of the coagulase gene and the x-region of spa. However, the dru region (direct repeat units) of mec-associated DNA exhibits a length polymorphism due to deletion of one or more direct repeat units. Five different subclones could be discriminated by dru region length polymorphism. Location of deletions and of a few point mutations allow a delineation of these subclones.

MeSH terms

  • Base Sequence
  • DNA, Bacterial / chemistry*
  • Methicillin Resistance*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Repetitive Sequences, Nucleic Acid*
  • Staphylococcus aureus / classification*

Substances

  • DNA, Bacterial