Comparison of polymerase chain reaction on fresh tissue samples and fecal drops on filter paper for detection of Trypanosoma cruzi in Rhodnius prolixus

P L Dorn; J Flores; B Brahney; A Gutierrez; R Rosales; A Rodas; C Monroy

doi:10.1590/s0074-02762001000400010

Comparison of polymerase chain reaction on fresh tissue samples and fecal drops on filter paper for detection of Trypanosoma cruzi in Rhodnius prolixus

Mem Inst Oswaldo Cruz. 2001 May;96(4):503-5. doi: 10.1590/s0074-02762001000400010.

Authors

P L Dorn¹, J Flores, B Brahney, A Gutierrez, R Rosales, A Rodas, C Monroy

Affiliation

¹ Department of Biological Sciences, Loyola University, New Orleans, LA, USA. dorn@loyno.edu

PMID: 11391422
DOI: 10.1590/s0074-02762001000400010

Abstract

PCR detection of Trypanosoma cruzi in Rhodnius prolixus using fresh tissue or fecal drops on filter paper showed comparable results: 38.7% infection rate using the fresh tissue sample and 37.9% by dried fecal drop.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Electrophoresis, Agar Gel
Feces / parasitology*
Filtration
Insect Vectors / parasitology
Intestines / parasitology*
Paper
Polymerase Chain Reaction / methods*
Rhodnius / parasitology*
Trypanosoma cruzi / isolation & purification*