Allpahuayo virus: a newly recognized arenavirus (arenaviridae) from arboreal rice rats (oecomys bicolor and oecomys paricola) in northeastern peru

Virology. 2001 Jun 5;284(2):277-86. doi: 10.1006/viro.2000.0803.

Abstract

Allpahuayo virus was initially isolated from arboreal rice rats (Oecomys bicolor and Oecomys paricola) collected during 1997 at the Allpahuayo Biological Station in northeastern Peru. Serological and genetic studies identified the virus as a new member of the Tacaribe complex of the genus Arenavirus. The small (S) segment of the Allpahuayo virus prototype strain CLHP-2098 (Accession No. AY012686) was sequenced, as well as that of sympatric isolate CLHP-2472 (Accession No. AY012687), from the same rodent species. The S segment was 3382 bases in length and phylogenetic analysis indicated that Allpahuayo is a sister virus to Pichinde in clade A. Two ambisense, nonoverlapping reading frames were identified, which result in two predicted gene products, a glycoprotein precursor (GPC) and a nucleocapsid protein (NP). A predicted stable single hairpin secondary structure was identified in the intergenic region between GPC and NP. Details of the genetic organization of Allpahuayo virus are discussed.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arenavirus / genetics
  • Arenavirus / immunology
  • Arenavirus / isolation & purification*
  • Base Sequence
  • Complement Fixation Tests
  • DNA, Intergenic
  • Genome, Viral
  • Glycoproteins / genetics
  • Molecular Sequence Data
  • Nucleocapsid / genetics
  • Peru
  • Phylogeny
  • Serotyping
  • Sigmodontinae / virology*
  • Viral Envelope Proteins / genetics

Substances

  • DNA, Intergenic
  • Glycoproteins
  • Viral Envelope Proteins

Associated data

  • GENBANK/AY012686
  • GENBANK/AY012687