Double-strand break repair: are Rad51/RecA--DNA joints barriers to DNA replication?

Trends Genet. 2001 Jun;17(6):318-21. doi: 10.1016/s0168-9525(01)02309-5.

Abstract

The central step of homologous recombination is the DNA strand exchange reaction catalyzed by bacterial RecA or eukaryotic Rad51. Besides Rad51-mediated synthesis-dependent strand annealing (SDSA), DNA ends can promote replication in Escherichia coli (recombination-dependent replication, RDR) and yeast (break-induced replication, BIR). However, what causes a DNA end to be repaired via SDSA or via BIR/RDR? I propose that Rad51/RecA--DNA plectonemic joints act as barriers to DNA replication and that BIR/RDR is only possible when the DNA polymerase that synthesizes DNA from the invading 3' end does not encounter RecA/Rad51--DNA joints in its path.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • DNA / chemistry
  • DNA / genetics
  • DNA Damage
  • DNA Nucleotidyltransferases / metabolism
  • DNA Repair*
  • DNA Replication*
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / genetics
  • Rad51 Recombinase
  • Rec A Recombinases / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins

Substances

  • DNA-Binding Proteins
  • Saccharomyces cerevisiae Proteins
  • DNA
  • DNA Nucleotidyltransferases
  • RAD51 protein, S cerevisiae
  • Rad51 Recombinase
  • Rec A Recombinases