Induction and characterization of hypoxanthine-phosphoribosyltransferase (Hprt-) deficient cell lines of Akodon cursor (Rodentia, Sigmodontinae)

Cytogenet Cell Genet. 2001;92(1-2):153-6. doi: 10.1159/000056888.

Abstract

Hypoxanthine-phosphoribosyltransferase negative (Hprt-) cell lines derived from an Akodon cursor liposarcoma were obtained by induced mutagenesis. All but one Hprt- cell line lacked Hprt mRNA transcripts while one (AKO 3) coded for a truncated protein. Cell fusion and karyotypic analyses showed that one cell line (AKO 1-15) could be successfully used for constructing hybrid panels and allow for a clear identification of human chromosomes in hybrid cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aneuploidy
  • Animals
  • Cell Fusion
  • Chromosomes / genetics*
  • Humans
  • Hybrid Cells / cytology*
  • Hybrid Cells / enzymology*
  • Hybrid Cells / metabolism
  • Hypoxanthine Phosphoribosyltransferase / chemistry
  • Hypoxanthine Phosphoribosyltransferase / deficiency*
  • Hypoxanthine Phosphoribosyltransferase / genetics
  • Karyotyping
  • Liposarcoma / enzymology
  • Liposarcoma / genetics
  • Liposarcoma / pathology
  • Lymphocytes / cytology
  • Lymphocytes / metabolism
  • Muridae / genetics*
  • Mutagenesis / genetics*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Deletion / genetics
  • Tumor Cells, Cultured

Substances

  • RNA, Messenger
  • Hypoxanthine Phosphoribosyltransferase