Abstract
Rsd (regulator of sigma D) is an anti-sigma factor for the Escherichia coli RNA polymerase sigma(70) subunit. The contact site of Rsd on sigma(70) was analyzed after mapping of the contact-dependent cleavage sites by Rsd-tethered iron-p-bromoacetamidobenzyl EDTA and by analysis of the complex formation between Ala-substituted sigma(70) and Rsd. Results indicate that the Rsd contact site is located downstream of the promoter -35 recognition helix-turn-helix motif within region 4, overlapping with the regions involved in interaction with both core enzyme and sigma(70) contact transcription factors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alanine
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Amino Acid Sequence
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Amino Acid Substitution
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Binding Sites
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DNA-Directed RNA Polymerases / genetics*
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DNA-Directed RNA Polymerases / metabolism
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli Proteins*
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Molecular Sequence Data
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Mutation
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Protein Binding
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Repressor Proteins / genetics*
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Repressor Proteins / isolation & purification
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Repressor Proteins / metabolism
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Sensitivity and Specificity
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Sigma Factor / genetics*
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Sigma Factor / metabolism
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Transcription, Genetic
Substances
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Escherichia coli Proteins
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Repressor Proteins
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Rsd protein, E coli
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Sigma Factor
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RNA polymerase sigma 70
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DNA-Directed RNA Polymerases
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Alanine