Oxidative stress increases MICA and MICB gene expression in the human colon carcinoma cell line (CaCo-2)

K Yamamoto; Y Fujiyama; A Andoh; T Bamba; H Okabe

doi:10.1016/s0304-4165(01)00099-x

Oxidative stress increases MICA and MICB gene expression in the human colon carcinoma cell line (CaCo-2)

Biochim Biophys Acta. 2001 Apr 3;1526(1):10-2. doi: 10.1016/s0304-4165(01)00099-x.

Authors

K Yamamoto¹, Y Fujiyama, A Andoh, T Bamba, H Okabe

Affiliation

¹ Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Otsu, Japan. yamak@belle.shiga-med.ac.jp

PMID: 11287116
DOI: 10.1016/s0304-4165(01)00099-x

Abstract

The human major histocompatibility complex class I chain-related A gene (MICA) and the MICB gene are newly identified members of the major histocompatibility complex class I chain-related gene family. We demonstrate here that oxidative stress, induced by H(2)O(2), promoted MICA (2.2-fold) and MICB (3.8-fold) gene expression using the human colon carcinoma cell line (CaCo-2) and semi-quantitative RT-PCR.

MeSH terms

Caco-2 Cells / metabolism*
Gene Expression Regulation
Histocompatibility Antigens Class I / biosynthesis*
Histocompatibility Antigens Class I / genetics
Humans
Hydrogen Peroxide
Membrane Proteins / metabolism*
Oxidative Stress*
Reverse Transcriptase Polymerase Chain Reaction

Substances

Histocompatibility Antigens Class I
MHC class I-related chain A
MICB antigen
Membrane Proteins
Hydrogen Peroxide