Modified nucleosides and bizarre 5'-termini in mouse myeloma mRNA

Nature. 1975 May 1;255(5503):28-33. doi: 10.1038/255028a0.

Abstract

Messenger RNAs from mouse myeloma cells contain N-6-methyl adenosine and novel 5' termini having 7-methy-guanosine in a 5', 5' triphosphate linkage with ribose-methylated nucleosides. Ten common 5'-terminal sequences of the forms m-7-G-5'-PPP-5'-NmpNp and m-7-G-5'-ppp-5'-NmpNmpNp are reported. Structures like this may be a general feature of mRNA in eukaryotes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenine Nucleotides / analysis
  • Adenosine / analysis
  • Animals
  • Autoradiography
  • Base Sequence
  • Cell Line
  • Electrophoresis
  • Guanosine / analysis
  • Mice
  • Multiple Myeloma / analysis*
  • Nucleosides / analysis*
  • Polynucleotides / analysis
  • RNA, Messenger / analysis*
  • RNA, Neoplasm / analysis*
  • RNA, Ribosomal / analysis
  • RNA, Transfer / analysis

Substances

  • Adenine Nucleotides
  • Nucleosides
  • Polynucleotides
  • RNA, Messenger
  • RNA, Neoplasm
  • RNA, Ribosomal
  • Guanosine
  • RNA, Transfer
  • Adenosine