Thyrotropin-releasing hormone and its receptor in glia

Glia. 2001 Mar 15;33(4):267-76. doi: 10.1002/1098-1136(20010315)33:4<267::aid-glia1025>3.0.co;2-1.

Abstract

The presence of thyrotropin-releasing hormone (Thyroliberin, TRH) and its receptor (TRH-R) in frozen coronal sections of the adult rat spinal cord and neonatal rat astroglial cultures was investigated by means of immunocytochemistry and Western blot using polyclonal antibodies generated against the hormone and monoclonal antibodies originated against discrete sequences of the type 1 rat TRH receptor (TRH-R1). TRH-R1 and TRH are present both in astroglial cells from adult rats and in cultured cells from newborn animals. The localization of TRH and TRH-R1 in nonneuronal cells in the central nervous system may reflect that some of the neurotrophic actions of TRH upon the central nervous system are mediated by glial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Astrocytes / chemistry*
  • Astrocytes / cytology
  • Blotting, Western
  • Cells, Cultured
  • Cerebral Cortex / chemistry
  • Cerebral Cortex / cytology
  • Immunohistochemistry
  • Male
  • Rats
  • Rats, Wistar
  • Receptors, Thyrotropin-Releasing Hormone / analysis*
  • Receptors, Thyrotropin-Releasing Hormone / immunology
  • Spinal Cord / chemistry
  • Spinal Cord / cytology
  • Thyrotropin-Releasing Hormone / analysis*
  • Thyrotropin-Releasing Hormone / immunology

Substances

  • Antibodies, Monoclonal
  • Receptors, Thyrotropin-Releasing Hormone
  • Thyrotropin-Releasing Hormone