[Differential accumulation of the new high-affinity phosphate transporter candidated gene fragment in rice roots in response to phosphorus deficiency stress]

Abstract

Phosphate is a major constraint to crop production, and phosphate uptake in plant is mainly by high-affinity phosphate transporter under phosphate deficiency condition. Using RT-PCR, a 1,178 bp phosphate transporter gene fragment OjPT1 was cloned from roots of Jingxi17 (Oryza sativa L. ssp. japanica) supplied with no phosphate. The comparison of this sequence with ones in GenBank indicated that it shared about 70% similarity at amino acid level with other phosphate transporters in higher plants, such as Arabidopsis thaliana, potate, tamato, Medicago truncatula and Catharanthus roseus, and high similarity with phosphate transporters in Saccharomyces cerevisiae and Neurospora crassa. RT-PCR assay showed that the OjPT1 transcripts were induced under phosphate deficiency condition. This gene fragment OjPT1 has been deposited in GenBank (accession No. AF249619).