Somatostatin blockade improves the proliferative response but not intestinal morphological growth after small bowel resection in rats

Eur J Surg. 2001 Jan;167(1):54-9. doi: 10.1080/110241501750069837.

Abstract

Objective: To find out whether or not blockade of somatostatin improves intestinal adaptation after small bowel resection.

Design: Laboratory experiment.

Setting: Teaching hospital, Spain.

Subjects: Eighty adult Wistar rats.

Interventions: Animals underwent intestinal resection or sham operation (n = 40 each) and were treated with a somatostatin antagonist either intermittently or continuously in three different doses (n = 8 each).

Main outcome measures: Bowel mucosal thickness, proliferation and concentrations of cAMP, somatostatin, insulin-like growth factor 1.

Results: Intestinal resection induced a proliferative and morphometric increase of the mucosa; however, the antagonist increased proliferation only in those animals given the highest dose. Intermittent doses induced a proliferative effect that was stronger than that in the three continuous groups. There was no relationship between trophic stimulus and insulin-like growth factor 1 or cAMP, but somatostatin concentrations increased after the intermittent course.

Conclusions: Somatostatin receptor blockade with an antagonist does not cause in normal rats an intestinal morphological adaptation process or increase it after resection; however, it did promote a proliferative stimulus in the crypts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight
  • Insulin-Like Growth Factor I / physiology
  • Intestinal Mucosa / physiology*
  • Intestine, Small / physiology*
  • Intestine, Small / surgery
  • Rats
  • Rats, Wistar
  • Receptors, Somatostatin / physiology
  • Somatostatin / antagonists & inhibitors
  • Somatostatin / physiology*

Substances

  • Receptors, Somatostatin
  • Somatostatin
  • Insulin-Like Growth Factor I