To investigate the conditions of endothelialization of biomaterials, we examined in vitro the growth properties of endothelial cells on PC treated yak pericardial materials. After rinsed in normal saline (0.9%), the surface of PC crosslinking yak pericardial materials was precoated with three kinds of adhesive proteins--Laminin (La), Fibronectin(FN) and Collagen-I(CL-I). Ednothelial cells(EC) from the canine were seeded on the precoated materials, and on the uncoated materials as control. The result showed that in the La and FN groups the seeded EC survived and grew on the patches, while in the CL-I group and uncoated group the seeded EC on and around the patches did not continue to live. Conclusively, in this experiment, PC yak pericardial materials had cytotoxicity; the cytotoxicity of PC crosslinking yak pericardial materials could be decreased by La and FN precoating; La and FN promoted the adhesion and growth of EC on the PC yak pericardial materials patches; the materials met the needs of endothelialization in vitro, prominently in the La group; there was significant difference in endothelialization between La and FN groups (P < 0.05); CL-I had no part in promoting EC adhesion and growth.