Purification of EEA1 from bovine brain cytosol using Rab5 affinity chromatography and activity assays

Methods Enzymol. 2001:329:120-32. doi: 10.1016/s0076-6879(01)29073-9.

Authors

S Christoforidis¹, M Zerial

Affiliation

¹ Laboratory of Biological Chemistry, Medical School, University of Ioannina, Ioannina 45110, Greece.

PMID: 11210528
DOI: 10.1016/s0076-6879(01)29073-9

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / cytology
Brain Chemistry*
Cattle
Chromatography, Affinity / methods*
Chromatography, Gel / methods
Chromatography, Ion Exchange / methods
Cytosol / chemistry*
Electrophoresis, Polyacrylamide Gel
Membrane Proteins / isolation & purification*
Membrane Proteins / metabolism*
Transfection
Vesicular Transport Proteins
rab5 GTP-Binding Proteins / genetics
rab5 GTP-Binding Proteins / metabolism*

Substances

Membrane Proteins
Vesicular Transport Proteins
early endosome antigen 1
rab5 GTP-Binding Proteins