Electrooxidation of phenol by catalase immobilized on graphite electrodes

Bioelectrochemistry. 2001 Jan;53(1):11-6. doi: 10.1016/s0302-4598(00)00089-1.

Abstract

Electrocatalytic properties of catalase (CAT) immobilized on graphite and soot to mediate electrooxidation of phenol have been investigated. The kinetic parameters--Km, k(s), deltaG*Ar and Z0 of the process studied were calculated. Conclusions on a probable mechanism of the biocatalytic and electrochemical process observed were drawn from the calculated values of activation and kinetic parameters. A quantitative UV-spectrophotometrical approach was used as an analytical tool. The electrochemical oxidation of phenol was examined with potentiodynamic and polarization curves' method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalase / metabolism*
  • Electrochemistry / methods
  • Electrodes
  • Enzymes, Immobilized / metabolism
  • Graphite
  • Kinetics
  • Oxidation-Reduction
  • Penicillium chrysogenum / enzymology
  • Phenol / chemistry
  • Phenol / metabolism*
  • Thermodynamics

Substances

  • Enzymes, Immobilized
  • Phenol
  • Graphite
  • Catalase