Neuroprotective constituents from Hedyotis diffusa

J Nat Prod. 2001 Jan;64(1):75-8. doi: 10.1021/np000327d.

Abstract

In a bioassay-guided search for neuroprotective compounds from medicinal plants, a MeOH extract of whole plants of Hedoytis diffusa yielded five flavonol glycosides, kaempferol 3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-D-galactopyranoside (1), quercetin 3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-D-galactopyranoside (2), quercetin 3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-D-glucopyranoside (3), kaempferol 3-O-(2-O-beta-D-glucopyranosyl)-beta-D-galactopyranoside (4), and quercetin 3-O-(2-O-beta-D-glucopyranosyl)-beta-D-galactopyranoside (5), and four O-acylated iridoid glycosides (6-9). Compounds 1 and 2 are previously unreported natural products, and all nine compounds exhibited significant neuroprotective activity in primary cultures of rat cortical cells damaged by L-glutamate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Survival / drug effects
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • Flavonoids / chemistry
  • Flavonoids / isolation & purification*
  • Flavonoids / pharmacology
  • Glycosides / chemistry
  • Glycosides / isolation & purification*
  • Glycosides / pharmacology
  • Kaempferols*
  • Magnetic Resonance Spectroscopy
  • Neurons / drug effects
  • Neuroprotective Agents / chemistry
  • Neuroprotective Agents / isolation & purification*
  • Neuroprotective Agents / pharmacology
  • Plants, Medicinal / chemistry*
  • Quercetin / analogs & derivatives
  • Quercetin / isolation & purification
  • Quercetin / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Rubiaceae / chemistry*
  • Spectrometry, Mass, Fast Atom Bombardment
  • Spectrophotometry, Ultraviolet

Substances

  • Flavonoids
  • Glycosides
  • Kaempferols
  • Neuroprotective Agents
  • kaempferol
  • Quercetin