Abstract
Neospora caninum infection is the major cause of bovine abortion. To develop a vaccine against N. caninum infection, recombinant vaccinia viruses carrying NcSRS2 and NcSAG1 genes (vv/Nc-p43 and vv/Nc-p36, respectively) were constructed and were tested in a mouse model. Vaccination of dams with vv/Nc-p43 appeared to confer effective protection against vertical transmission to offspring, though that with vv/Nc-p36 only provided partial protection. Moreover, the vv/Nc-p43 vaccination provoked cellular immune responses and antibody production against N. caninum. In conclusion, it is expected that vv/Nc-p43 can be used as an effective live vaccine to prevent vertical transmission of N. caninum in natural hosts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antibodies, Protozoan / immunology
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Antibody Specificity
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Antigens, Protozoan / genetics
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Antigens, Protozoan / immunology
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Coccidiosis / immunology
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Coccidiosis / parasitology
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Coccidiosis / prevention & control*
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Coccidiosis / transmission
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Concanavalin A / pharmacology
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DNA, Protozoan / analysis
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Female
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Genes, Protozoan / genetics*
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Infectious Disease Transmission, Vertical*
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Litter Size
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Male
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Mice
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Mice, Inbred BALB C
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Neospora / genetics
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Neospora / immunology*
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Pregnancy
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Protozoan Vaccines / genetics
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Protozoan Vaccines / immunology*
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Spleen / drug effects
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Spleen / immunology
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Survival Rate
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T-Lymphocytes / immunology
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Vaccination
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology*
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Vaccinia virus / genetics*
Substances
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Antibodies, Monoclonal
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Antibodies, Protozoan
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Antigens, Protozoan
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DNA, Protozoan
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Protozoan Vaccines
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Vaccines, Synthetic
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Concanavalin A