Abstract
A gene coding for a Fur (ferric uptake regulation) protein from the cyanobacterium Anabaena PCC 7119 has been cloned and overexpressed in Escherichia coli. DNA sequence analysis confirmed the presence of a 151-amino-acid open reading frame that showed homology with the Fur proteins reported for the unicellular cyanobacteria Synechococcus 7942 and Synechocystis PCC 6803. Two putative Fur-binding sites were detected in the promoter regions of the fur gene from Anabaena. Partially purified recombinant Fur binds to the flavodoxin promoter as well as its own promoter. This suggests that the Fur gene is autoregulated in Anabaena.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anabaena / genetics*
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Cloning, Molecular
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DNA, Bacterial / analysis
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Escherichia coli
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Flavodoxin / genetics
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Molecular Sequence Data
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Promoter Regions, Genetic* / genetics
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Promoter Regions, Genetic* / physiology
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Repressor Proteins / biosynthesis
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Sequence Homology, Nucleic Acid
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Transfection
Substances
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Bacterial Proteins
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DNA, Bacterial
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Flavodoxin
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Recombinant Proteins
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Repressor Proteins
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ferric uptake regulating proteins, bacterial