Rapid identification of virulent type I strains of the protozoan pathogen Toxoplasma gondii by PCR-restriction fragment length polymorphism analysis at the B1 gene

J Clin Microbiol. 2001 Jan;39(1):398-400. doi: 10.1128/JCM.39.1.398-400.2001.

Abstract

Sequence analysis at the 35-fold-repetitive B1 locus identified three restriction sites capable of discriminating type I (mouse-virulent) from type II or III (mouse-avirulent) strains of Toxoplasma gondii. B1 PCR-restriction fragment length polymorphism analysis of 8 type I, 17 type II, and 8 type III strains confirms the specificity of the assay. It should now be possible to ask whether strain genotype affects the severity and type of clinical disease in humans.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA, Protozoan / genetics
  • Genes, Protozoan*
  • Genotype
  • Humans
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Restriction Fragment Length
  • Protozoan Proteins / genetics*
  • Sequence Analysis, DNA
  • Toxoplasma / classification*
  • Toxoplasma / genetics
  • Toxoplasma / pathogenicity*
  • Toxoplasmosis / parasitology*
  • Virulence / genetics

Substances

  • DNA, Protozoan
  • Protozoan Proteins