The aim of our study was the evaluation of the anatomy of ulnar and median nerves in the upper limb in order to ameliorate knowledge on the clinical anatomy of these nerves. In fact, further information on this topic may be useful owing to its possible clinical relevance when planning surgical anatomy and reconstructive surgery in tumor affected and injured patients. The relationships between ulnar and median nerve and neighbouring anatomical structures have been examined, together with the course and ramification of the ulnar and median nerves in six fresh cadavers. Moreover, we have performed a review of the literature. Four specific aspects were evaluated during dissection: 1) division modality of the ulnar nerve at the wrist; 2) anatomical details of the medial humeral epicondyle; 3) anatomical relationships between median nerve and retinaculum flexorum; 4) median-ulnar nerves anastomosis. Our results show that: the medial humeral epicondyle shows specific anatomical details in relation to the ulnar nerve; the relationships between the median nerve and the transverse carpal ligament may be characterized by one or two nerve trunks (two cases of bifid median nerve in our experience); median-ulnar nerve anastomosis may be also found at various levels. Comparing our results with those of the available literature we can conclude that anatomical variations of ulnar and median nerve in the upper limb are not an infrequent finding and their clinical, diagnostic and surgical relevance should be considered.