The novel isoform of the progesterone receptor cDNA in the human testis and detection of its mRNA in the human uterine endometrium

Oncology. 2000:59 Suppl 1:39-44. doi: 10.1159/000055286.

Abstract

A novel isoform (termed isoform S) of the progesterone receptor (PR) cDNA (PR isoform S cDNA) which consists of a previously unidentified 5' sequence and exons 4-8 of the intracellular PR gene has been cloned from the human testicular cDNA library. The 5' sequence of the message was confirmed to be derived from a novel exon (termed exon S) by genomic cloning. The expression level of the PR isoform S mRNA was higher in the spermatozoon than in the uterine endometrium with a lower expression level of the PR isoforms B and A mRNAs in the spermatozoon than in the endometrium. These results implied that the PR isoform S which was possibly translated from the PR isoform S mRNA in the spermatozoon might be related to the cell surface membrane PR. Moreover, the PR isoform S in the uterine endometrium might play some physiological and/or pathogenic roles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • DNA, Complementary / chemistry*
  • Endometrium / chemistry*
  • Female
  • Humans
  • Isomerism
  • Male
  • Molecular Sequence Data
  • RNA, Messenger / analysis*
  • Receptors, Progesterone / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Testis / chemistry*

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Progesterone