Blue-fluorescent antibodies

Science. 2000 Oct 13;290(5490):307-13. doi: 10.1126/science.290.5490.307.

Abstract

The forte of catalytic antibodies has resided in the control of the ground-state reaction coordinate. A principle and method are now described in which antibodies can direct the outcome of photophysical and photochemical events that take place on excited-state potential energy surfaces. The key component is a chemically reactive optical sensor that provides a direct report of the dynamic interplay between protein and ligand at the active site. To illustrate the concept, we used a trans-stilbene hapten to elicit a panel of monoclonal antibodies that displayed a range of fluorescent spectral behavior when bound to a trans-stilbene substrate. Several antibodies yielded a blue fluorescence indicative of an excited-state complex or "exciplex" between trans-stilbene and the antibody. The antibodies controlled the isomerization coordinate of trans-stilbene and dynamically coupled this manifold with an active-site residue. A step was taken toward the use of antibody-based photochemical sensors for diagnostic and clinical applications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Catalytic / chemistry*
  • Antibodies, Monoclonal / chemistry*
  • Binding Sites
  • Binding Sites, Antibody
  • Chemical Phenomena
  • Chemistry, Physical
  • Crystallography, X-Ray
  • Fluorescence*
  • Haptens
  • Ligands
  • Microscopy, Fluorescence
  • Models, Chemical
  • Models, Molecular
  • Photochemistry
  • Spectrometry, Fluorescence
  • Stereoisomerism
  • Stilbenes / chemistry*
  • Stilbenes / immunology*
  • Temperature
  • Ultraviolet Rays

Substances

  • Antibodies, Catalytic
  • Antibodies, Monoclonal
  • Haptens
  • Ligands
  • Stilbenes

Associated data

  • PDB/19G2-2