Clean Western blots of membrane proteins after yeast heterologous expression following a shortened version of the method of Perini et al

Anal Biochem. 2000 Oct 15;285(2):276-8. doi: 10.1006/abio.2000.4784.
No abstract available

MeSH terms

  • Animals
  • Blotting, Western / methods*
  • Calcium-Transporting ATPases / analysis*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Gene Expression
  • Mercaptoethanol / pharmacology
  • Rats
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Sodium Dodecyl Sulfate / pharmacology
  • Yeasts / metabolism

Substances

  • Sodium Dodecyl Sulfate
  • Mercaptoethanol
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Calcium-Transporting ATPases