2'-Deoxy-8-(propyn-1-yl)adenosine-containing oligonucleotides: effects on stability of duplex and quadruplex structures

B Catalanotti; A Galeone; L Gomez-Paloma; L Mayol; A Pepe

doi:10.1016/s0960-894x(00)00381-4

2'-Deoxy-8-(propyn-1-yl)adenosine-containing oligonucleotides: effects on stability of duplex and quadruplex structures

Bioorg Med Chem Lett. 2000 Sep 4;10(17):2005-9. doi: 10.1016/s0960-894x(00)00381-4.

Authors

B Catalanotti¹, A Galeone, L Gomez-Paloma, L Mayol, A Pepe

Affiliation

¹ Dip. di Chimica delle Sostanze Naturali, Univ. di Napoli Federico II, Italy.

PMID: 10987437
DOI: 10.1016/s0960-894x(00)00381-4

Abstract

2'-Deoxy-8-(propyn-1-yl)adenosine has been incorporated in synthetic oligodeoxyribonucleotides and its influence on thermal stability of duplex and quadruplex structures investigated by UV, CD and 1H NMR. The obtained results seem to indicate that the presence of the modified base negatively affects the stability of double stranded DNA whereas remarkably increases the stability of parallel quadruplex structures.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine
Circular Dichroism
DNA / chemistry*
Magnetic Resonance Spectroscopy
Nucleic Acid Conformation
Oligodeoxyribonucleotides / chemistry*

Substances

Oligodeoxyribonucleotides
DNA
Adenosine