Soft tissue Ewing sarcoma--peripheral primitive neuroectodermal tumor with atypical clear cell pattern shows a new type of EWS-FEV fusion transcript

Diagn Mol Pathol. 2000 Sep;9(3):137-44. doi: 10.1097/00019606-200009000-00003.

Abstract

This study describes a new case of Ewing sarcoma (ES)-peripheral primitive neuroectodermal tumor (pPNET) with unusual phenotype and fusion gene structure. The tumor located in the inguinal area of a 15-year-old boy showed a highly aggressive behavior with hematogenous metastases after intensive chemotherapy and bone marrow transplant, causing death 28 months after diagnosis. The tumor displayed a clear cell pattern, and several neuroectodermal markers proved positive both in the original tumor and in xenografts. This neuroectodermal character was confirmed by electron microscopy. Moreover, cytogenetically the tumor has an unusual chromosomal rearrangement, t(2;22)(q13;q22,t(3;18)(p21;q23); representing a new EWS-FEV fusion type in which exon 7 of EWS gene is fused with exon 2 of FEV gene. This is the third published study of an ES-pPNET showing EWS-FEV fusion described, but it is the first study of a tumor with the aforementioned fusion points. These findings support the genetic and morphologic heterogeneity existing within the group of ES-pPNET tumors.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adolescent
  • Animals
  • Chromosomes, Human, Pair 2 / genetics*
  • Chromosomes, Human, Pair 2 / ultrastructure
  • Chromosomes, Human, Pair 22 / genetics*
  • Chromosomes, Human, Pair 22 / ultrastructure
  • Combined Modality Therapy
  • Disease Progression
  • Exons / genetics
  • Fatal Outcome
  • Groin
  • Humans
  • Karyotyping
  • Male
  • Mice
  • Mice, Nude
  • Neoplasm Metastasis
  • Neoplasm Proteins / analysis
  • Neuroectodermal Tumors, Primitive / genetics*
  • Neuroectodermal Tumors, Primitive / pathology
  • Oncogene Proteins, Fusion / genetics*
  • Prognosis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sarcoma, Ewing / genetics*
  • Sarcoma, Ewing / pathology
  • Soft Tissue Neoplasms / genetics*
  • Soft Tissue Neoplasms / pathology
  • Translocation, Genetic / genetics*
  • Transplantation, Heterologous
  • Tumor Cells, Cultured

Substances

  • EWS-FEV fusion protein, human
  • Neoplasm Proteins
  • Oncogene Proteins, Fusion