Isolation and characterization of low-indole-3-acetic acid-producing mutants from Bradyrhizobium elkanii

K Yagi; T Matsumoto; T Chujo; H Nojiri; T Omori; K Minamisawa; M Nishiyama; H Yamane

doi:10.1271/bbb.64.1359

Isolation and characterization of low-indole-3-acetic acid-producing mutants from Bradyrhizobium elkanii

Biosci Biotechnol Biochem. 2000 Jul;64(7):1359-64. doi: 10.1271/bbb.64.1359.

Authors

K Yagi¹, T Matsumoto, T Chujo, H Nojiri, T Omori, K Minamisawa, M Nishiyama, H Yamane

Affiliation

¹ Biotechnology Research Center, The University of Tokyo, Japan.

PMID: 10945251
DOI: 10.1271/bbb.64.1359

Abstract

We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bradyrhizobium / genetics
Bradyrhizobium / isolation & purification
Bradyrhizobium / metabolism*
DNA, Bacterial / analysis
Indoleacetic Acids / metabolism*
Mutagenesis
Restriction Mapping / methods
Transposases

Substances

DNA, Bacterial
Indoleacetic Acids
indoleacetic acid
Transposases