ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan

FEBS Lett. 2000 Aug 4;478(3):241-5. doi: 10.1016/s0014-5793(00)01854-8.

Abstract

A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu(1871)-Leu(1872) bond within the chondroitin sulfate attachment domain of aggrecan. In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADAM Proteins
  • ADAMTS1 Protein
  • Aggrecans
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Cartilage / chemistry*
  • Cattle
  • Chondroitin Sulfates / metabolism
  • Disintegrins / genetics
  • Disintegrins / metabolism*
  • Extracellular Matrix Proteins*
  • Lectins, C-Type
  • Metalloendopeptidases / genetics
  • Metalloendopeptidases / metabolism*
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Mutation / genetics
  • Peptide Fragments / chemistry
  • Peptide Fragments / genetics
  • Peptide Fragments / metabolism
  • Proteoglycans / chemistry
  • Proteoglycans / genetics
  • Proteoglycans / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Analysis, Protein
  • Substrate Specificity

Substances

  • Acan protein, mouse
  • Aggrecans
  • Disintegrins
  • Extracellular Matrix Proteins
  • Lectins, C-Type
  • Peptide Fragments
  • Proteoglycans
  • Recombinant Fusion Proteins
  • Chondroitin Sulfates
  • ADAM Proteins
  • ADAMTS1 Protein
  • Adamts1 protein, mouse
  • Metalloendopeptidases