Isolation and characterization of neural stem cells from the adult human olfactory bulb

Stem Cells. 2000;18(4):295-300. doi: 10.1634/stemcells.18-4-295.

Abstract

We have recently isolated stem cells deriving from the olfactory bulbs of adult patients undergoing particularly invasive neurosurgery. After improving our experimental conditions, we have now obtained neural stem cells according to clonal analysis. The cells can be expanded, established in continuous cell lines and differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes). Also, after exposition to leukemia inhibitory factor, we are able to improve the number of neurons, an ideal biological source for transplantation in various neurodegenerative disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Astrocytes / cytology*
  • Astrocytes / drug effects
  • Cell Count
  • Cell Culture Techniques / methods
  • Cell Separation
  • Cells, Cultured
  • Growth Inhibitors / pharmacology
  • Humans
  • Interleukin-6*
  • Leukemia Inhibitory Factor
  • Lymphokines / pharmacology
  • Neurons / cytology*
  • Neurons / drug effects
  • Olfactory Bulb / cytology*
  • Oligodendroglia / cytology*
  • Oligodendroglia / drug effects
  • Stem Cells / cytology*
  • Stem Cells / drug effects

Substances

  • Growth Inhibitors
  • Interleukin-6
  • LIF protein, human
  • Leukemia Inhibitory Factor
  • Lymphokines