Comparative evaluation of a field-based dot-ELISA kit with three other serological tests for the detection of Brucella antibodies in goats

Trop Anim Health Prod. 2000 Jun;32(3):155-63. doi: 10.1023/a:1005283531306.

Abstract

A dot-ELISA (d-ELISA) test was evaluated and compared with the serum agglutination test (SAT), micro-complement fixation test (CFT) and a plate-ELISA (p-ELISA) for field use in screening herds of goats against brucellosis. During the standardization of the dot-ELISA kit on 1732 caprine serum samples, 1571 samples out of 1666 were found to be negative in d-ELISA, SAT and micro-CFT, while 59 were positive in different combinations. Of a further 66 serum samples, 34 were negative and 31 were positive in different combinations in d-ELISA, SAT, micro-CFT and p-ELISA. A total of 1584 goats belonging to different herds were then screened for brucellosis. Of the 694 serum samples screened in the first batch using d-ELISA, a positive reaction was observed in 26 cases. Further screening of these cases revealed 13 and 21 goats as positive reactors in SAT and CFT, respectively. In a second batch of 890 goats there were 109 positive reactors in d-ELISA. Among these 109 goats, 34, 40 and 80 goats were positive reactors in SAT, CFT and p-ELISA, respectively. The results of d-ELISA correlated well with those of p-ELISA. Dot-ELISA was found to be a more suitable and rapid test for screening large numbers of goats in the field.

Publication types

  • Comparative Study

MeSH terms

  • Agglutination Tests / veterinary
  • Animals
  • Antibodies, Bacterial / blood
  • Brucella / immunology*
  • Brucella / isolation & purification
  • Brucellosis / blood
  • Brucellosis / diagnosis
  • Brucellosis / veterinary*
  • Complement Fixation Tests / veterinary
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Goat Diseases / diagnosis*
  • Goat Diseases / microbiology
  • Goats
  • Reagent Kits, Diagnostic / veterinary

Substances

  • Antibodies, Bacterial
  • Reagent Kits, Diagnostic