Isoproterenol-induced myocardial injury resulting in altered S100A4 and S100A11 protein expression in the rat

Pathol Int. 2000 Jun;50(6):480-5. doi: 10.1046/j.1440-1827.2000.01069.x.

Abstract

S-100 proteins (S100) are characterized by calcium-binding ability with two structural EF hands. Several S100 are expressed in cardiomyocytes and thought to play a crucial role in calcium signaling. To examine whether the expression of S100 is a response to detectable myocardial damage or regeneration, we investigated, immunohistochemically, the expression of S100A4 and S100A11 in the isoproterenol (ISP)-treated rat heart. Definite expression of S100A4 and S100A11 was demonstrated in normal cardiomyocytes, and their staining patterns were enhanced in the ISP-treated rat heart, suggesting the possible involvement of S1-A4 and S100A11 in ISP-induced myocardial damage.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Cardiomegaly / chemically induced
  • Cardiomegaly / metabolism*
  • Cardiomegaly / pathology
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Heart Ventricles / chemistry
  • Heart Ventricles / drug effects
  • Heart Ventricles / pathology
  • Humans
  • Immune Sera / immunology
  • Immunohistochemistry
  • Isoproterenol / adverse effects*
  • Male
  • Molecular Sequence Data
  • Myocardium / chemistry
  • Myocardium / pathology
  • Rats
  • Rats, Wistar
  • S100 Calcium-Binding Protein A4
  • S100 Proteins / analysis*
  • S100 Proteins / genetics
  • S100 Proteins / immunology
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Immune Sera
  • S100 Calcium-Binding Protein A4
  • S100 Proteins
  • S100a4 protein, rat
  • S100A4 protein, human
  • S100A11 protein, human
  • Isoproterenol