IGF-II and IL-2 act synergistically to alter HDAC1 expression following treatments with trichostatin a

Cytokine. 2000 Jul;12(7):1104-9. doi: 10.1006/cyto.2000.0680.

Abstract

Histone deacetylases play key roles in the regulation of gene transcription. Studies have shown that expression of interleukins IL-2 and IL-8, and insulin-like growth factor 2 (IGF2) are affected by treatment with histone deacetylase inhibitors. We have previously shown that the gene for histone deacetylase 1 (HDAC1) is upregulated following treatment with TSA. The murine homologue of this gene has been reported to be inducible by IL-2. In this study, we have examined the effects IL-2, IGF-II and TSA have on HDAC1 expression in the human hepatocellular carcinoma derived cell line Hep3B. Our results indicate that in contrast to the mouse, HDAC1 is not inducible by IL-2. However, in TSA treated cells, IL-2 and IGF-II were found to act synergistically to reduce TSA induced HDAC1 mRNA levels almost to normal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Animals
  • Drug Synergism
  • Enzyme Inhibitors / pharmacology*
  • Flow Cytometry / methods
  • Histone Deacetylase 1
  • Histone Deacetylase Inhibitors*
  • Histone Deacetylases / biosynthesis*
  • Histones / metabolism
  • Humans
  • Hydroxamic Acids / pharmacology*
  • Insulin-Like Growth Factor II / pharmacology*
  • Interleukin-2 / pharmacology*
  • Mice
  • Tumor Cells, Cultured

Substances

  • Enzyme Inhibitors
  • Histone Deacetylase Inhibitors
  • Histones
  • Hydroxamic Acids
  • Interleukin-2
  • trichostatin A
  • Insulin-Like Growth Factor II
  • HDAC1 protein, human
  • Histone Deacetylase 1
  • Histone Deacetylases