An in-gel assay of a recombinant western corn rootworm (Diabrotica virgifera virgifera) cysteine proteinase expressed in yeast

Anal Biochem. 2000 Jun 15;282(1):153-5. doi: 10.1006/abio.2000.4591.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cockroaches / enzymology
  • Cystatins / pharmacology
  • Cysteine Endopeptidases / chemistry*
  • Cysteine Endopeptidases / genetics
  • Cysteine Endopeptidases / isolation & purification*
  • Cysteine Proteinase Inhibitors / analysis
  • Enzyme Precursors / analysis
  • Mutagenesis
  • Pichia / enzymology
  • Pichia / metabolism*
  • Protein Isoforms
  • Recombinant Proteins / analysis
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / metabolism
  • Soybean Proteins
  • Transcription, Genetic

Substances

  • Cystatins
  • Cysteine Proteinase Inhibitors
  • Enzyme Precursors
  • Protein Isoforms
  • Recombinant Proteins
  • Soybean Proteins
  • scN protein, soybean
  • Cysteine Endopeptidases