A highly sensitive, non-radioactive assay for T cell activation in cattle: applications in screening for antigens recognised by CD4(+) and CD8(+) T cells

J Immunol Methods. 2000 May 26;239(1-2):85-93. doi: 10.1016/s0022-1759(00)00187-3.

Abstract

We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNgamma and activation of immune CD4(+) and CD8(+) T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4(+) T cell clones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Protozoan / immunology
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology
  • Cattle
  • Cell Division
  • Cell Line
  • Ehrlichia ruminantium / immunology*
  • Endothelium, Vascular / cytology
  • Histocompatibility Antigens Class I / immunology
  • Histocompatibility Antigens Class II / biosynthesis
  • Interferon-gamma / pharmacology
  • Iodine Radioisotopes
  • Lymphocyte Activation / immunology*
  • Recombinant Proteins
  • Sensitivity and Specificity
  • T-Lymphocytes, Cytotoxic / cytology
  • T-Lymphocytes, Cytotoxic / immunology*
  • Theileria parva / immunology*

Substances

  • Antigens, Protozoan
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Iodine Radioisotopes
  • Recombinant Proteins
  • Interferon-gamma