Molecular cloning and characterization of a novel human gene (NESCA) which encodes a putative adapter protein containing SH3

Biochim Biophys Acta. 2000 Apr 25;1491(1-3):321-6. doi: 10.1016/s0167-4781(00)00049-x.

Abstract

A full-length cDNA encoding a novel protein was isolated and sequenced from a human placental cDNA library. This cDNA consists of 1990 bp and has a predicted open reading frame encoding 433 amino acids. It possesses an Src homology 3 (SH3) motif, a leucine zipper motif and no catalytic domain, suggesting that it seems to be an adapter protein. PCR-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 1q21-22.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • COS Cells
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • Gene Library
  • Genes*
  • Humans
  • Leucine Zippers
  • Molecular Sequence Data
  • Placenta / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Transfection
  • src Homology Domains

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • DNA, Complementary
  • RUSC1 protein, human

Associated data

  • GENBANK/AB026894